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MBA DNA Consulting, LLC - Los Angeles, CA

Forensic DNA Consulting Blog

Multiple Injections of Same Sample-Capillary Electrophoresis

Multiple Injections of Same Sample-Capillary Electrophoresis

I was recently asked about how electropherograms appear if the same sample vial is injected multiple times on an instrument.  In order to hopefully provide a helpful example, I have scanned some actual data that I have obtained in the past.

The data below are from the repeated 5s injection of a 9947A sample on a single capillary  ‘310’  instrument  (Blue Identifiler loci shown) run as a part of a precision study.

9947A precision

The  data below are from 5s and 10s injections of the same, low level DNA sample on the same 310 run (all Identifiler loci shown)

5s10s

Here is some additional data showing 5s and 10s injections of the same sample on the same 310 run (Identifiler)

addl5s10s

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